- __Bloom Laboratory
Movement of Isolated Golgi Membranes Along Microtubules (Real time)
Reference: Fullerton, A.T., M.-Y. Bau, P.A. Conrad. and G.S. Bloom. 1998. In Vitro Reconstitution of Microtubule Plus End-directed, GTPWS-Sensitive Motility of Golgi Membranes. Molecular Biology of the Cell: 2699 2714.)
Small Golgi fragments, which have a vesicle-like appearance, can be seen to move along microtubules during the early stages (~13 minutes) of a control experiment.
This sequence highlights motility of Golgi tubules along microtubules at a later time point (~43 minutes) of a control experiment.
Inhibition of motility by
is illustrated in this movie, which was recorded ~82 minutes after
the start of the experiment. 1 µM
was present throughout the entire experiment, during which time Golgi
fragments rarely moved, and only a few, short Golgi tubules
Movies from unpublished work (Real time)
CHO cells stably transfected with caveolin-1-GTP, a marker for caveolae. Note the movement of caveolae along linear tracks, which correspond to microtubules. Time compression ~73X; movie recorded by Dorothy Mundy.
CV-1 cell transiently transfected with YFP-IQGAP1. Note the presence of the fluorescent fusion protein in lamellipodia and ruffles. Time compression ~75X; recorded by Sue Lin.