CURRICULUM VITAE
John Thomas Parsons
Birthday: May 15, 1942
Birthplace: Chicago, Illinois
Marital status: Married to Sarah Anne Jox, June 20, 1964
Children: Scott Thomas and Rebecca Anne
Home address: 2818 Millington Road, Charlottesville, VA 22901
Education: B.A., Chemistry, 1964
DePauw University, Greencastle, Indiana
Ph.D., Biochemistry, 1968
Duke University, Durham, North Carolina
Research training:
1963 NSF Undergraduate Research Program, Department of Chemistry, DePauw University.
1964-1966 USPHS Predoctoral Trainee, Department of Biochemistry, Duke University, laboratory of Dr. Kenneth McCarty.
1966-1968 USPHS Predoctoral Fellowship, Department of Biochemistry, Duke University, Dr. Kenneth McCarty.
1968-1970 American Cancer Society Postdoctoral Fellowship, Institute for Molecular Virology, St. Louis University School of Medicine, laboratory of Dr. Maurice Green.
1970-1973 Assistant, Institut fur Molekularbiologie, Universitat Zurich, Zurich, Switzerland; laboratory of Dr. Charles Weissmann.
Academic positions:
1973-1974 Oberassistant, Institut fur Molekularbiologie, Universitat Zurich, Zurich, Switzerland.
1974-1980 Assistant Professor, Department of Microbiology, University of Virginia.
1980-1984 Associate Professor, Department of Microbiology, University of Virginia.
1984-present Professor, Department of Microbiology, University of Virginia.
1992-present F. Palmer Weber Medical Research Professor
1992-1994 Associate Director for Basic Research, U. Virginia Cancer Center
1994-present Chairman, Department of Microbiology.
Editorial appointments:
Editorial Board (past, present): Journal of Virology, Virology , Cell Adhesion and Communication, Journal of Biological Chemistry, Journal of Cell Science, Cellular Microbiology.
Professional societies:
American Society for Microbiology
American Society for Virology
American Society of Biological Chemists
American Society of Cell Biology
Principal teaching duties:
Department of Microbiology: Graduate course in Molecular Pathogenesis (one semester course), Medical Microbiology, lecture and laboratory.
Administrative duties and committees (past and present):
University of Virginia: University Biohazard Committee, Recombinant DNA Subcommittee, Biological Hazards Subcommittee.
University of Virginia Faculty Forum for Scientific Research.
University of Virginia Faculty Senate.
University of Virginia Cancer Center Advisory Committee.
University of Virginia, Molecular Biology Institute/Markey Center Steering Committee.
Search Committees: Markey Center Director, Physiology Chair, Directorship of the Thaler Center for HIV and AIDS Research (Chairman).
American Cancer Society: Microbiology and Virology Advisory Committee Ad Hoc 1979, 1981; permanent member, 1983-1987; Personnel Committee, 1989-1991, Vice-Chairman, 1990, 91; Chairman, 1992.
NIH Public Advisory Committees, Experimental Virology Study Section, 1990-1994, Chairman, 1992-94.
Program Project Site-Visit Review Committees (National Cancer Institute), 1983, 1984, 1985, 1987, 1988, 1989, 1990, 1991, 1992, 1995, Committee Chairman, 1988, 1989, 1990, 1992 and 1993, 1996, NCI Program Project Parent Review Committee , 1996-2000 .
NIEHS Review Committee, Ad Hoc member 1987.
State of Virginia, Center for Innovative Technology, Biotechnology Institute Advisory Committee, 1985-1988.
External Consultant: Purdue Cancer Center, 1994-present.
External Consultant: University of Kentucky Cancer Center 1997-present.
Current research support:
DHHS-NCI 5 R37 CA29243. 01/01/81-04/30/00. Avian tumor virus antigens. (Direct Cost: $186,410)
DHHS-NCI P01 CA 40042. 09/30/85-02/28/99. Program Project: Oncogenes and Mitogens:Intracellular Mechanisms. Program Director. (Direct Cost: $686,809)
DHHS-NCI P01 CA 40042. 09/30/85-02/28/99. Program Project: Oncogenes and Mitogens:Intracellular Mechanisms. Project 2: Genetic Analysis of the Structure and Function of a Cellular Tyrosine Protein Kinase. (Direct Cost: $163,023)
CTR4491. The Council for Tobacco Research. 07/01/96-06/30/99. Novel regulation of focal adhesion kinase. (Direct Cost: $92,000)
CaP CURE 01/01/98-12/31/99 Adhesion signaling: A target for novel therapeutic drugs. (Direct Cost: $100,000).
Publications:
1. McCarthy, K.S., J.T. Parsons, W.A. Carter, and J. Laszlo. 1966. Protein-synthetic capacities of liver nuclear subfractions. J. Biol. Chem. 241: 5489-5499.
2. Parsons, J.T., and K.S. McCarty. 1968. Rabidly-labeled messenger ribonucleic acid-protein complex of rat liver nuclei. J. Biol. Chem. 243: 5377-5384.
3. Green, M., M. Rokutunda, K. Fujinaga, H. Rokutunda, C. Gurgo, R.K. Ray, and J.T. Parsons. 1970. Synthesis of DNA by RNA tumor viruses and viral RNA by virus transformed cells. The biology of oncogenic viruses. 1970 Lepetit Symposium on Oncogenic Viruses.
4. Green, M., J.T. Parsons, H. Caffier, M. Landgraf-Leurs, and D. Tsuei. 1970. Transcription of adenovirus genes in productively infected and in transformed cells. The biology of oncogenic viruses. 1970 Lepetit Symposium on Oncogenic Viruses.
5. Green, M., J.T. Parsons, M. Pina, K. Fujinaga, H. Caffier, and I. Landgraf-Leurs. 1970. Transcription of adenovirus genes in productively infected and in transformed cells. Cold Spring Harbor Symposium on Quantitative Biology 35: 803-818.
6. Fujinaga, K., J.T. Parsons, J.W. Beard, D. Beard, and M. Green. 1970. Mechanism of carcinogenesis by RNA tumor viruses. III. Formation of RNA-DNA complex and duplex DNA molecules by the DNA polymerase(s) of avian myeloblastosis virus. Proc. Natl. Acad. Sci. USA 67: 1432-1439.
7. Caffier, H., H.J. Raskas, J.T. Parsons, and M. Green. 1971. In vitro and in vivo initiation of mammalian viral protein synthesis. Nature New Biology 229: 239-241.
8. Parsons, J.T., J. Gardner, and M. Green. 1971. Biochemical studies on adenovirus multiplication. XIX. Resolution of late viral RNA species in the nucleus and cytoplasm. Proc. Natl. Acad. Sci. USA 68: 557-560.
9. Parsons, J.T., J.M. Coffin, R.K. Haroz, P.A. Bromley, and C.Weissmann. 1973. Quantitative determination and location of newly synthesized virus-specific RNA in chicken cells infected with Rous sarcoma virus. J. Virol. 11: 761-774.
10. Parsons, J.T., and M. Green. 1971. Biochemical studies on adenovirus multiplication. XVIII. Resolution of early virus-specific RNA species in AD 2 infected and transformed cells. Virology 45: 154-162.
11. Weissmann, C., J.T. Parsons, J.W. Coffin, L. Rymo, M.A. Billeter, and H. Hofstetter. 1974. Studies on the structure and synthesis of Rous sarcoma virus RNA. Cold Spring Harbor Symposium on Quantitative Biology 39: 1046-1056.
12. Coffin, J.M., J.T. Parsons, L. Rymo, R.K. Haroz, and C. Weissmann. 1974. A new approach to the isolation of RNA-DNA hybrids and its application to the quantitative determination of labeled tumor virus RNA. J. Mol. Biol. 86: 373-396.
13. Rymo, L., J.T. Parsons, J.M. Coffin, and C. Weissmann. 1974. In vitro synthesis of Rous sarcoma virus-specific RNA is catalyzed by a DNA-dependent RNA polymerase. Proc. Natl. Acad. Sci. USA 71: 2782-2786.
14. Billeter, M.A., J.T. Parsons, and J.M. Coffin. 1974. The nucleotide sequence complexity of avian tumor virus RNA. Proc. Natl. Acad. Sci. USA 71: 3560-3564.
15. Collett, M., P. Dierks, and J.T. Parsons. 1977. In vitro synthesis of unique RNA species by a T particle of vesicular stomatitis virus. J. Virol. 23: 708-716.
16. Collins, C.J., and J.T. Parsons. 1977. Integration of avian sarcoma virus DNA sequences in transformed mammalian cells. Proc. Natl. Acad. Sci. USA 74: 4301-4305.
17. Leis, J.P., R.E. Smith, P. Dierks, J.T. Parsons, M.S. Collett, and A.J. Faras. 1978. In vitro transcription of reconstituted 35S RNA × tRNAtrp template × primer complexes by the avian oncornavirus DNA polymerase: Effect of temperature on the size of the DNA transcripts. Virology 85: 28-42.
18. Collett, M., P. Dierks, J.T. Parsons, and A.J.Faras. 1978. RNase H hydrolysis of the 5' terminus of the avian sarcoma virus genome during reverse transcription. Nature 272: 181-183.
19. Emerson, S.U., P. Dierks, and J.T. Parsons. 1977. RNA Synthesis by a defective particle of vesicular stomatitis virus. In Negative Strand Viruses (Mahy, B.W.J., and Barry, R.D., eds.), Academic Press, London.
20. Parsons, J.T., P. Lewis, and P. Dierks. 1978. Purification of virus-specific RNA from chicken cells infected with avian sarcoma virus: Identification of genome-length and subgenome-length viral RNAs. J. Virol. 27: 227-238.
21. Parsons, S.J., S. Riley, E. Mullen, E. Brock, D.C.Benjamin, W.M.Kuehl, and J.T. Parsons. 1979. Immune response to the src gene product in mice bearing tumors induced by injection of avian sarcoma virus transformed mouse cells. J. Virol. 32: 40-46.
22. Dierks, P.M., P.E. Highfield, and J.T. Parsons. 1979. Deletion mutant of Bratislava-77 Strain rous sarcoma virus containing a fusion of the group specific antigen and envelope genes. J. Virol. 32: 567-582.
23. Gilmer, T.M.,and Parsons, J.T. 1979. Analysis of cellular integration sites in avian sarcoma virus-infected duck embryo cells. J. Virol. 32: 762-769.
24. Collins, C.J., D. Boettiger, T.L. Green, M.B. Burgess, B.H. Devlin, and J.T. Parsons. 1980. Arrangement of integrated avian sarcoma virus DNA sequences within the cellular genomes of transformed and revertant mammalian cells. J. Virol. 33: 760-768.
25. Highfield, P.E., L.F. Rafield, T.M. Gilmer, and J.T. Parsons. 1980. Molecular cloning of avian sarcoma virus closed circular DNA: Structural and biological characterization of three recombinant clones. J. Virol. 36: 271-279.
26. Omer, C.A., J.T. Parsons, and A.J. Faras. 1981. Elucidation of the 5'-3' transcriptional jump during reverse transcription. J. Virol. 38: 398-402.
27. Gilmer, T.M., J.T. Parsons, and R.L.Erikson. 1982. Construction of plasmids for expression of Rous sarcoma virus transforming protein, pp60src, in Escherichia coli. Proc. Natl. Acad. Sci. USA 79: 2152-2156.
28. Mirsra, T.K., D.P. Grandgenett, and J.T. Parsons. 1982. Avian retrovirus pp32 DNA binding protein I. Recognition of specific sequences on retrovirus DNA terminal repeats. J. Virol. 44: 330-343.
29. Bryant, D., and J.T. Parsons. 1982. Site-directed mutagenesis of the src gene of Rous sarcoma virus: Construction and characterization of a deletion mutant temperature-sensitive for transformation. J. Virol. 44: 683-691.
30. Bryant, D., and J.T. Parsons. 1983. Site-directed point mutant in the src gene of RSV results in an inactive src gene product. J. Virol. 45: 1211-1216.
31. Parsons, S.J., L.A. Daigle, C.M. Ely, J.T. Parsons, and D.C. Benjamin. 1984. Monoclonal antibodies specific for the virion polypeptide P27 of avian retroviruses. Hybridoma 3: 25-31.
32. Parsons, S.J., D.J. McCarley, C.M. Ely, D.C. Benjamin, and J.T.Parsons. 1983. Isolation and partial characterization of a monoclonal antibody to the Rous sarcoma virus transforming protein pp60src. J. Virol. 45: 1190-1194.
33. Gilmartin, G., and J.T.Parsons. 1983. Identification of transcriptional elements within the long terminal repeat of Rous sarcoma virus. Mol. Cell. Biol. 3: 1834-1845.
34. Parsons, J.T., D.L. Bryant, V. Wilkerson, G. Gilmartin, and S.J. Parsons. 1984. Site-directed mutagenesis of rous sarcoma virus pp60src: Identification of functional domains required for transformation. Cancer Cells, Oncogenes and Viral Genes 2: 37-42.
35. Bryant, D.L., and J.T. Parsons. 1984. Amino acid alterations within a highly conserved region of the Rous sarcoma virus src gene product pp60src inactive protein kinase activity. Mol. Cell. Biol. 4: 862-866.
36. Parsons, S.J., D.J. McCarley, C.M. Ely, D.C. Benjamin, and J.T. Parsons. 1984. Monoclonal antibodies to Rous sarcoma virus pp60v-src are reactive with enzymatically active pp60c-src of avian and mammalian origin. J. Virol. 51: 272- 282.
37. Wilkerson,V., D.L. Bryant, and J.T. Parsons. 1985. Rous sarcoma virus variants that encode src proteins with an altered carboxy terminus are defective for transformation. J. Virol. 55: 314-321.
38. Morgan, J.H., T.S. Papas, and J.T. Parsons. 1985. Isolation of antibodies specific for avian viral and cellular myc proteins. J. Natl. Can. Inst. 75: 937-947.
39. Chen, W.T., J.M. Chen, S.J. Parsons, and J.T. Parsons. 1985. Local degradation of fibronectin at sites of expression of the transforming gene product pp60src. Nature 316: 156-158.
40. Parsons, J.T., V. Wilkerson, and S.J. Parsons. 1986. Structural and functional motifs of the Rous sarcoma virus src protein. Gene Amplification and Analysis 4: 1-19.
41. Morgan, J.H., and J.T. Parsons. 1986. Characterization of c-myc proteins from avian bursal lymphoma cell lines. Virology 150: 178-186.
42. Heaney, M., J.H. Pierce, and J.T. Parsons. 1986. Site directed mutagenesis of the gag-myc gene of avian myelocytomatosis virus 29: Biological activity and intracellular localization of structurally altered proteins. J. Virol. 60: 167-176.
43. Parsons, S.J., and J.T. Parsons. 1986. Monoclonal antibodies to oncogene products: Derivation and uses. In Monoclonal Antibody Hybridoma Techniques (L. Shook, ed.), Marcel Dekker, New York, pp. 207-228.
44. Parsons, S.J., D.J. McCarley, V.W. Raymond, and J.T. Parsons. 1986. Localization of conserved and nonconserved epitopes within the Rous sarcoma virus encoded src protein. J. Virol. 59: 755-758.
45. McCarley, D.J., C.M. Ely, D.C. Benjamin, J.T. Parsons, and S.J. Parsons. 1987. Inhibition of pp60v-src, pp70v-fgr and pp90v-yes tyrosine kinase activity by a monoclonal antibody specific for the amino terminus of pp60pp60v-src. J. Virol. 61: 1927-1937.
46. Raymond, W.V., and J.T. Parsons. 1987. Identification of an amino terminal domain required for the transforming activity of the Rous sarcoma virus src protein. Virology 160: 400-410.
47. Biegalke, B.J., M.L. Heaney, A., Bouton, J.T. Parsons, and M. Linial. 1987. MC29 deletion mutants which fail to transform chicken macrophages are competent for transformation of quail macrophages. J. Virol. 61: 2138-2142.
48. Taparowsky, B.J., M.L. Heaney, and J.T. Parsons. 1987. Oncogene mediated multistep transformation of C3H10T1/2 cells. Can. Res. 47: 4125-4129.
49. Reynolds, A. B., J. Vila, T. J. Lansing, W. M. Potts, M. J. Weber, and J. T. Parsons. 1987. Activation of the oncogenic potential of the avian cellular src protein by specific structural alteration of the carboxy terminus. EMBO J. 6: 2359-2364.
50. Kanner, S.B., S.J. Parsons, J.T. Parsons, and T.M. Gilmer. 1988. Activation of pp60c-src tyrosine kinase specific activity in tumor-derived syrian hamster embryo cells. Oncogene 2: 327-335.
51. Potts, W.M., Reynolds, A.R., Lansing, T.J., and Parsons, J.T. 1988. Activation of pp60c-src transforming potential by mutations altering the structure of an amino terminal domain containing residues 90-95. Oncogene Res. 3: 343-355 .
52. Kanner, S.B., Gilmer, T.M., Reynolds, A.B., and Parsons, J.T. 1989. Novel tyrosine phosphorylations accompany the activation of pp60c-src during chemical carcinogenesis. Oncogene 4: 295-300.
53. Wang, H.-C. R., and Parsons, J.T. 1989. Deletions and insertions within an amino-terminal domain of pp60v-src inactivate transformation and modulate membrane stability. J. Virol. 63: 291-302.
54. Reynolds, A.B., Roesel, D.J., Kanner, S.B., and Parsons, J.T. 1989. Transformation-specific tyrosine phosphorylation of a novel cellular protein in chicken cells expressing oncogenic variants of the avian cellular src gene. Mol. Cell. Biol. 9: 629-638.
55. Parsons, J.T., and M.J. Weber. 1989. Genetics of src: Structure and function of a protein tyrosine kinase. Curr. Topics Microbiol. Immunol. 147: 80-127.
56. Wilson, L.K., D.K. Luttrell, J.T. Parsons, and S.J. Parsons. 1989. Pp60c-src tyrosine kinase, myristylation and modulatory domains are required for the enhanced mitogenic responsiveness to epidermal growth factor seen in cells overexpressing c-src. Mol. Cell. Biol. 9: 1536-1544.
57. Kanner, S.B., A.B. Reynolds, and J.T. Parsons. 1989. Immunoaffinity purification of tyrosine-phosphorylated cellular proteins. J. Immunol. Methods. 120: 115-124.
58. Reynolds, A.B., S.B. Kanner, H.-C. R. Wang, and J.T. Parsons. 1989. Stable association of activated pp60src with two tyrosine phosphorylated cellular proteins. Mol. Cell. Biol. 9: 3951-3958.
59. Kanner, S.B., A.B. Reynolds, R.R. Vines, and J.T. Parsons. 1990. Monoclonal antibodies to individual tyrosine-phosphorylated protein substrates of oncogene-encoded tyrosine kinases. Proc. Natl. Acad. Sci. USA. 87: 3328-3332.
60. Parsons, J.T. 1990. Closing the gap in a signal transduction pathway. Trends in Genetics. 6: 169-171.
61. Kanner, S.B., A.B. Reynolds, and J.T. Parsons. 1991. Tyrosine phosphorylation of 120-kilodalton pp60src substrate upon epidermal growth factor and platelet-derived growth factor receptor stimulation and in polyomavirus middle-T-transformed cells. Mol. Cell. Biol. 11: 713-720.
62. Bouton, A.H., S.B. Kanner, R.R. Vines, H.-C. R. Wang, J.B. Gibbs, and J.T. Parsons. 1991. Transformation by pp60src or stimulation of cells with epidermal growth factor induces the stable association of tyrosine phosphorylated cellular proteins with GTPase activating protein. Mol. Cell. Biol. 11: 945-953.
63. Kanner, S.B., A.B. Reynolds, H-C.R. Wang, R.R. Vines, and J.T. Parsons. 1991. The SH2 and SH3 domains of pp60src direct stable association with tyrosine phosphorylated proteins p130 and p110. EMBO J. 10: 1689-1698.
64. Wu, H., A.B. Reynolds, S.B. Kanner, R.R. Vines, and J.T.Parsons. 1991. Identification and characterization of a novel cytoskeleton-associated pp60src substrate. Mol. Cell. Biol. 11: 5113-5124.
65. Bouton, A.H., S.B. Kanner, R.R. Vines, and J.T. Parsons. 1991. Tyrosine phosphorylation of three cellular proteins correlates with transformation of Rat 1 cells by pp60src. Mol. Carcinogen. 4: 145-152.
66. Cobb, B.S., D.M.Payne, A.B. Reynolds and J.T. Parsons. 1991. Regulation of the oncogenic activity of the cellular src protein requires the correct spacing between the kinase domain and the C-terminal phosphorylated Tyrosine (Tyr-527). Mol. Cell. Biol. 11: 5832-5838.
67. Flynn, D.C., M.D. Schaller, and J.T. Parsons. 1992. Tyrosine phosphorylation of a 120 000 dalton membrane-associated protein by the neural form of pp60c-src, pp60c-src+. Oncogene 7: 579-583.
68. Schaller, M.D., C.A.Borgman, B.S. Cobb, R.R. Vines, A.B. Reynolds, and J.T. Parsons. 1992. PP125FAK, a structurally distinctive protein tyrosine kinase associated with focal adhesions. Proc. Natl. Acad. Sci. USA, 89: 5192-5196.
69. Maa, M-C., L.K. Wilson, J.S. Moyers, R.R. Vines, J.T. Parsons, and S.J. Parsons. 1992. P75, an EGF sensitive substrate of the c-src tyrosine kinase. Oncogene 7: 2429-2438.
70. Lipfert, L., B. Haimovich, M.D. Schaller, B.S. Cobb, J.T. Parsons, J.S. Brugge, 1992. Integrin-dependent phosphorylation and activation of the protein tyrosine kinase pp125FAK in platelets. J. Cell Biol. 119: 905-912.
71. Bouton, A.H. and J.T. Parsons. 1992. Retrovirus and cancer: models for cancer in animals and man. Cancer Investigation. 11: 70-79.
72. Farina, S.F., J.L. Huff, and J.T. Parsons. 1992. Mutations within the 5' half of the avian retrovirus MC29 v-myc gene alter or abolish transformation of chicken embryo fibroblasts and macrophages. J. Virol. 66: 2698-2708.
73. Kornberg, L., H. S. Earp, J.T. Parsons, M.D. Schaller, and R.L. Juliano. 1992. Cell adhesion or integrin clustering increases phosphorylation of a focal adhesion associated tyrosine kinase. J. Biol. Chem. 267: 23439-23442.
74. Ely, C.M., S.J. Parsons, and J.T. Parsons. 1992. Immunological methods for the detection of phosphotyrosine containing proteins in neural tissues. Neuroprotocols 1: 177-184.
75. Parsons, J.T. and S.J. Parsons. 1992. Protein tyrosine kinases and their relationship to oncogenes. Advances in Oncology (Eds: Devita, V.T., Hellman, S., and Rosenberg, S.A.) J. B. Lippincott. pp 3-17.
76. Guy, C.T., M.A. Webster, M.D.Schaller, J.T. Parsons, R.D. Cardiff and W.J. Muller. 1992. Expression of the c-neu proto-oncogene in the mammary epithelium of transgenic mice induces metastatic disease. Proc. Natl. Acad. Sci. USA. 89: 10578-10582.
77. Schaller, M.D., C.A. Borgman, and J.T. Parsons. 1993. Autonomous expression of a noncatalytic domain of the focal adhesion associated protein tyrosine kinase pp125FAK. Mol Cell. Biol. 13: 789-791.
78. Wu, H. and J.T. Parsons. 1993. Cortactin, an 80/85-killodalton pp60src substrate , is a filamentous actin-binding protein enriched in the cell cortex. J. Cell Biol. 120: 1417-1426.
79. Schaller, M.D., A.H. Bouton, D.C. Flynn, and J.T. Parsons. 1993. Identification and characterization of novel substrates for protein tyrosine kinases. Prog. Nucl. Acids Res. and Mol. Biol. (eds: W.E.Cohn and K. Moldave) 44: 205-227.
80. Huff, J.L., M.A. Jelinek, C.A. Borgman, T.J. Lansing, and J.T. Parsons. 1993. The protooncogene c-sea encodes a transmembrane protein tyrosine kinase related to the MET/hepatocyte growth factor/scatter factor receptor. Proc. Natl. Acad. Sci. USA 90: 6140-6144.
81. Schaller, M.D. and J.T. Parsons. 1993. Focal adhesion kinase: an integrin-linked protein tyrosine kinase. Trends in Cell Biology. 3: 258-262.
82. Cobb, B.S. and J.T. Parsons. 1993. Regulation of the cellular src protein tyrosine kinase: interactions of the carboxyl terminal sequences residing between the kinase domain and tyrosine-527. Oncogene. 8: 2897-2903.
83. Turner, C.E., M.D. Schaller, and J.T. Parsons. 1993. Tyrosine phosphorylation of the focal adhesion kinase pp125FAK during development: relation to paxillin. J. Cell Science 105: 637-645.
84. Hildebrand, J.D., M.D. Schaller and J.T. Parsons. 1993. Identification of sequences required for the efficient localization of the focal adhesion kinase,pp125FAK, to cellular focal adhesions. J. Cell Biol. 123: 993-1005.
85. Flynn, D.C., T-H. Leu, A.B. Reynolds and J.T. Parsons. 1993. Identification and sequence analysis of cDNAs encoding a 110 kilodalton actin filament-associated pp60src substrate. Mol. Cell. Biol. 13:7892-7900.
86. Faris, M., F. Gaskin, J.T. Parsons and S.M. Fu. 1994. CD40 signaling pathway: Anti-CD40 monoclonal antibody induces rapid dephosphorylation and phosphorylation of tyrosine-phosphorylated proteins including protein tyrosine kinase Lyn, Fyn, and Syk and the appearance of a 28-kD tyrosine phosphorylated protein. J. Exp. Med. 179:1923-1931.
87. Cobb, B.S., M.D. Schaller, T-H. Leu and J.T. Parsons. 1994. Stable association of pp60src and pp59fyn with the focal adhesion-associated protein tyrosine kinase, pp125FAK. Mol. Cell. Biol. 14 147-155.
88. Schaller, M.D., J.D. Hildebrand, J.D. Shannon, J.W. Fox, R.R. Vines, and J.T. Parrsons 1994. Autophosphorylation of the focal adhesion kinase, pp125FAK, directs SH2-dependent binding of pp60src. Mol. Cell. Biol. 14: 1680-1688.
89. Miglarese, M. R., J. Mannion-Henderson, H. Wu, J.T. Parsons, and T.P. Bender. 1994. The protein tyrosine kinase substrate cortactin is differently expressed in murine B lymphoid tumors. Oncogene 9: 1989-1997.
90. Shattil, S.J., B. Haimovich, M. Cunningham, L. Lipfert, J.T. Parsons, M. Ginsberg, and J. Brugge. 1994. Tyrosine phosphorylation of pp125FAK in platelets requires coordinated signalling through integrin and agonist receptors. J. Biol. Chem. 20: 14738-14745.
91. Schaller, M.D. and Parsons, J.T. 1994. Focal adhesion kinase and associated proteins. Current Opionion in Cell Biology 6: 705-710.
92. Marengere, L.E.M., Z. Songyang, G.D. Gish, M.D. Schaller, J.T. Parsons, M.J. Stern, L.C. Cantley, and T. Pawson. 1994. SH2 domain specificity and activity modified by a single residue. Nature 369: 502-505.
93. Parsons, J.T., M.D. Schaller, J. Hildebrand, T.-H. Leu, A. Richardson, and C. Otey. 1994. Focal adhesion kinase: structure and signalling. In Cell Biology of Cancer (Eds. David Glover, Alan Hall and Nick Hastie) J. Cell Sci. 18: 109-113.
94. Richardson, A. and J.T. Parsons. 1995. Review articles: Signal transduction through integrins: a central role for focal adhesion kinase? BioEssays 17: 229-236.
95. Schaller, M.D. and J.T.Parsons. 1995. pp125fak-dependent tyrosine phosphorylation of paxillin creates a high affinity binding site for Crk. Mol. Cell. Biol. 15: 2635-2645.
96. Schaller, M.D., C.A. Otey, J.D. Hildebrand, and J.T. Parsons. 1995. Focal adhesion kinase and paxillin bind to peptides mimicking ² integrin cytoplasmic domains. J. Cell. Biol. 130: 1181-1187.
97. Hildebrand, J.D., M.D. Schaller, and J.T. Parsons. 1995. Paxillin, a tyrosine phosphorylated focal adhesion-associated protein binds to the carboxylterminal domain of focal adhesion kinase. Mol. Bio. Cell. 6: 637-647.
98. Huff, J.L. and J.T. Parsons. 1995. SEA receptor PTK. The Protein Kinase Factsbook. (Eds. D.G. Hardy and S. Hanks). 221-222.
99. Huff, J.L., M.A. Jelinek, T.A. Jamieson and J.T. Parsons. 1996. Expression and maturation of the cellular sea receptor, a member of the hepatocyte growth factor (HGF) receptor family of protein tyrosine kinases. Oncogene 12: 299-307.
100. Parsons, J.T. 1996. Integrin-mediated signalling: regulation by protein tyrosine kinase and small GTP-binding proteins. Current Opinions in Cell Biology 8: 146-152.
101. Richardson, A. and J.T. Parsons. 1996. A mechanism for the regulation of the adhesion-associated protein tyrosine kinase pp125FAK. Nature 380: 538-540.
102. Medico, E., A.M. Mongiovi, J. Huff, M.A. Jelinek, A. Follenzi, G. Gaudino, J.T. Parsons and P.M. Comoglio. 1996. The tyrosine kinase receptors Ron and Sea control "scattering" and morphogenesis of liver progenitor cells in vitro. Mol. Biol. Cell. 7: 495-504.
103. Hildebrand, J.D., J.M. Taylor and J.T. Parsons. 1996. An SH3 domain-containing GTPase-activating protein for Rho and Cdc42 associates with focal adhesion kinase. Mol. Cell. Biol. 16: 3169-3178.
104. Harte, M.T., J.D. Hildebrand, M.R. Burnham, A.H. Bouton and J.T. Parsons. 1996. p130Cas, a substrate associated with v-Src and v-Crk, localizes to focal adhesions and binds to focal adhesion kinase. J. Biol. Chem. 271: 13649-13655.
105. Chen, H.-C., J.T. Parsons, J.D. Hildebrand, M.D. Schaller and J.-L. Guan. 1996. Interaction of FAK with cytoskeletal protein talin. Accepted for publication J. Cell. Biol. 270: 16995-16999.
106. Malik, R.J. and J.T. Parsons. 1996. Integrin-mediated signaling in normal and malignant cells: a role of protein tyrosine kinases. Biochim. Biophys. Acta 1287: 73-76.
107. Burnham, M.R., M.T. Harte, A. Richardson, J.T. Parsons and A.H. Bouton. 1996. The identification of p130cas-binding proteins and their role in cellular transformation. Oncogenes 12: 2467-2472.
108. Malik, R.K. and J.T. Parsons. 1996. Integrin-dependent activation of the p70 ribosomal S6 kinase signaling pathway. J. Biol. Chem. 271: 29785-29791.
109. Richardson, A., J.D. Shannon, M.D. Schaller, and J.T. Parsons. 1997. Identification of integrin-stimulated sites of serine phosphorylation in FRNK, the separately expressed C-terminal domain of focal adhesion kinase: a potential role for protein kinase A. Biochem. J. 324: 141-149.
110. Parsons, J.T. and S.J. Parsons. 1997. Src family protein tyrosine kinases: cooperating with growth factor and adhesion signaling pathways. Current Opinion in Cell Biology 9:187-192.
Published Textbooks:
Volk, W.A., D.C. Benjamin, R. Kadner, and J.T. Parsons. 1986. Essentials in Microbiology, Third Edition. J.B. Lippincott Company, Philadelphia.
Volk, W.A., D.C. Benjamin, R. Kadner, and J.T. Parsons. 1990. Essentials in Microbiology, Fourth Edition. J.B. Lippincott Company, Philadelphia.
Invited seminars and talks-1993
Keystone Symposium: Transcription factors, regulation and differentiation.
Midwinter Conference of Immunologists: Asilamar CA
Winter Gordon Conference on "Fibronectin and related molecules".
Summer Gordon Conference on "Cancer"
Summer Gordon Conference on "The molecular and genetic basis of cell proliferation".
American Society of Bone and Mineral Research, "Symposium on the Cell Matrix".
Carolina Conference on Integrins and Cell Signalling.
SUNY-Stony Brook, Department of Microbiology.
University of New Mexico, Symposium on "Cell Adhesion and Growth".
Centecor Inc.
University of Michigan, Department of Physiology.
-1994
Keystone Symposium on Cell Signalling.
M.D. Anderson, Brain Research Center; External consultant.
Penn State Medical University, students of the Cell Biology Program.
Medical School of the University of Maryland, Department of Microbiology.
Wesleyan University, Cell Biology Program.
British Cell Biology Society, Symposium on the Cancer Cell (Edinburgh, Scotland).
Fifth Workshop on Cells and Cytokines in Bones and Cartilage (Davos Switzerland).
University of Virginia, Department of Pathology.
University of Virginia, Department of Pharmacology.
Columbia University, Department of Pathology.
University of Pennsylvania, Department of Microbiology.
Purdue Cancer Center, external consultant.
Summer Gordon Conference on Growth Factor Receptors (August).
The Metastasis Society Meeting-Symposium on Cell Adhesion (September).
Schweppe Colloquium on Tumor Metastasis, Northwester University (September).
American Physiological Society: Symposium on Mechanotransduction and Regulation of Growth and Differentiation.
New York University Medical School, Department of Cell Biology.
Yale Medical School, Department of Cell Biology.
American Society of Cell Biology - Chair: Mini Symposium on Adhesion Signalling.
-1995
UCLA Workshop on Signal Transduction and Cancer.
Winter Gordon Conference on Fibronectin.
Co-Organizer American Cancer Society Meeting of Virginia Cancer Research.
University of Wisconsin, Department of Pharmacology.
Purdue Cancer Center, external consultant.
Co-Organizer, ONCOGENE Meeting, Frederick, MD.
Co-Organizer, Cell Signalling and the Cytoskeleton, Lake Placid, NY.
Jacque Monod Conference on Morphogenic functions of actin-associated proteins. Aussois, France.
Nobel Conference on Cell Adhesion Molecules and Cell Matrix Receptors in Vertebrate Tissues, Stockholm, Sweden
Mount Sinai Medical Center, Department of Microbiology.
St. Jude Research Hospital, Department of Cell Biology.
Becton Dickinson Symposium on Extracellular Matrix, Oxford, England
Harvard University Medical School, Signal Transduction Department.
-1996
Keystone Symposium on Integrins and Signaling Events in Cell Biology and Disease (session chair).
AAAS Symposium on Signal Transduction.
American Society of Neurochemists, Symposium on Regulation of Focal Adhesion Signaling.
Merck Research Laboratories.
University of Minnesota, Burroughs Welcome Visiting Professor, Department of Laboratory Medicine.
University of Indiana Medical School, Cancer Center.
NCI-Workshop on Prostate Cancer.
University of Massachusetts Medical School, Program in Molecular Medicine.
Purdue Cancer Center, external consultant.
FASEB Summer Conference on Signal Transduction.
Frederick Cancer Research Facility.
Texas Southwestern Medical School, Department of Pharmacology.
University of South Carolina, Basic Medical Sciences Program.
-1997.
University of Kentucky, external consultant.
Keystone Symposium on Integrin Signaling, invited speaker and session chair.
NIH-NCI, Seminar
University of North Carolina, Seminar, Neurobiology program
Rockefeller University, Teruko Hanafusa Memorial Symposium, invited speaker
Gordon Conference on the Molecular and Genetic Basis of Cell Proliferation, invited speaker and session chair
Purdue University Cancer Center, external consultant
Fourth Annual CaPCure Scientific Retreat, participant and speaker
-1998.
Keystone/Copper Mountain Symposium on Motility and Metastasis, invited speaker.
University of West Virginia Cancer Center.
NCI Workshop on functional imaging, Consultant.
FASEB/AAA Meetings, San Francisco; Mini Symposium on Cell Motility, invited speaker.
Chicago Signal Transduction Symposium, invited speaker.
Nobel Symposium on Viral and Microbial Pathogenesis, Stockholm; invited speaker.
University of Edinburgh, Center for Neurosciences, invited speaker.
Personnel trained/current personnel:
Postdoctoral Fellows: (27 total)
Dr. Carolyn J.Collins, 1976-1977, (Ph.D., Duke University); Research Associate Professor, Department of Pathology, Harvard Medical School.
Dr. Blythe Devlin, 1978-1979, (Ph.D. University of Virginia); Research Assistant Professor, Emory University.
Dr. Peter Highfield (with Dr.Kuehl) 1978-1980, (Ph.D., University of Bristol); Senior Research Scientist, Wellcome Laboratories, England.
Dr. Thea Pugatsch, 1979-1980, (Ph.D., ETH, Zurich); Research Associate, Roche Institute; Senior Scientist, International Genetic Sciences Partnership, Jerusalem, Israel.
Dr. Debra Bryant, 1980-1983, (Ph.D., Bowman Gray/ Wake Forest University); Research Scientist, Oncogene, Seattle, WA., presently, Humogen Inc., Charlottesville, Va.
Dr. Jon Morgan, 1982-1986. (Ph.D., Duke University); Research Scientist, FMC Corporation, Rockland, Maine.
Dr. Helen Kim, 1983-1985. (Ph.D., University of Virginia), Assistant Professor of Anatomy, University of Alabama.
Dr. Elizabeth Taparowsky, 1984-1986, (Ph.D. Brown University), Associate Professor, Purdue University.
Dr. William Potts, 1985-1988. (Ph.D., Cornell University); Research Scientist, Smith, Kline and Beckman.
Dr. Albert Reynolds, 1985-1989. (Ph.D., University of Virginia), Associate Professor, Department of Cell Biology, Vanderbilt University Medical School.
Dr. Steven Kanner, 1986-1990. (Ph.D., University of Miami), Research Scientist, Bristol-Myers Squibb.
Dr. Amy Bouton, 1986-1991. (Ph.D., University of Virginia), Assistant Professor, University of Virginia.
Dr. Daniel Flynn, 1988-1992. (Ph.D., North Carolina State University), Assistant Professor of Microbiology, West Virginia University.
Dr. Michael Schaller, 1990-1994, (Ph.D., McMaster University), Assistant Professor of Cell Biology and Anatomy, University of North Carolina, Chapel Hill.
Dr. Zheng Horng Leu, 1992-1994, (Ph.D., University of Virginia), Assistant Professor, Taiwan.
Dr. Alan Richardson, 1993-present, (Ph.D., Cambridge University, UK).
Dr. Mary Anne Jelinek, 1992-1995, (Ph.D., McMaster University), Research Scientist, Upstate Biotechnology Inc., Lake Placid, NY
Dr. Hong Wu, 1992-1994, (Ph.D., University of Virginia), Resident in Pathology, University of Pennsylvania.
Dr. Janice Huff, 1992-1994 (Ph.D., University of Virginia).
Dr. Trudy Marshall, 1994-1996, (Ph.D., University of Ottawa).
Dr. Wen-Cheng Xiong, 1994-present. (Ph.D. John Hopkins).
Dr. Judith LaCoste, 1994-present, (Ph.D., McGill University).
Dr. Mary Harte, 1994-1997, (Ph.D., Medical College of Ohio).
Dr. Joan Taylor, 1995-present, (Ph.D., University of Michigan).
Dr. Scott Weed, 1996-present, (Ph.D., Yale University).
Dr. Karin Martin, 1997-present, (Ph.D., Duke University).
Dr. Yoost Loijens, 1998-present (University of Wisconsin).
Graduate Students: (18 total)
John Cahill, M.A., 1977, Microbiologist, Food and Drug Administration, AAAS Congressional Fellow; Lawyer; Hazel, Beckhorn and Hanes.
Peter Dierks, Ph.D., 1979, Senior scientist, Cyanamide Inc., Princeton, NJ.
Lori Rafield, Ph.D.,1981, Biotechnology Consultant, San Francisco, CA.
Tona M.Gilmer, Ph.D.,1980, Group leader, Glaxo, Research Triangle, NC.
Greg Gilmartin, 1983, Assistant Professor, University of Vermont.
Victoria Wilkerson, 1986, Postdoctoral Fellow, University of North Carolina.
Robert Wang, 1990, Postdoctoral fellow, Harvard University, Assistant Professor, Ohio State Cancer Center.
Mark Heaney, 1986, Assistant Professor, Oncology, Sloan-Kettering Cancer Center.
Stephen Farina, 1991, Postdoctoral fellow, NIH.
Janice Huff: 1992, Postdoctoral fellow, University of Virginia.
Brad Cobb: 1992, Postdoctoral fellow, University of Chicago.
Timothy Jamieson, 1988, Resident in Oncology, Duke University.
Hong Wu, 1992, Resident in Pathology, University of Pennsylvania.
Jeff Hildebrand, 1995, Fred Hutchinson Cancer Research Center.
Yunrui Du, 1998, Yale Medical School.
Kate Nolan, Current graduate student.
Amy Ma, Current graduate student.
Andrew Kinley, Current graduate student